Rapid, label-free, contactless measurement of membrane potential in excitable H9c2 cardiomyoblasts using ζ-potential (2024)

Chacar, S., Catacutan, M. K., Albakr, S., Al Safar, H., Babiker, S., Ahmed, S., Albizreh, A. A., Alshehhi, A. Z., Lee, S., Nader, M. (2024). Rapid, label-free, contactless measurement of membrane potential in excitable H9c2 cardiomyoblasts using ζ-potential. Measurement Science and Technology, 35(5), Article 055701. https://doi.org/10.1088/1361-6501/ad25de

Chacar, Stephanie ; Catacutan, Mary Krystelle ; Albakr, Shamma et al. / Rapid, label-free, contactless measurement of membrane potential in excitable H9c2 cardiomyoblasts using ζ-potential. In: Measurement Science and Technology. 2024 ; Vol. 35, No. 5.

@article{f5d67c5d429042af9a3f6911fa07ede1,

title = "Rapid, label-free, contactless measurement of membrane potential in excitable H9c2 cardiomyoblasts using ζ-potential",

abstract = "The measurement of cell membrane potential (V m) is important for understanding ion channel function. V m plays a role in several routine cellular functions and diseases, particularly in excitable cells such as muscle and nerve. However, measuring V m is difficult, relying either on labour-intensive direct measurement of single cells (intracellular electrodes, patch clamp) or indirect measurement of fluorescence intensity, using V m-sensitive labels. Here we demonstrate a direct measurement technique based on determination of the cell{\textquoteright}s ζ-potential, the electrical potential at the hydrodynamic shear plane, approximately 1 nm beyond the cell surface. We demonstrate this principle using excitable H9c2 cardiomyoblasts, measured in both polarised and depolarised states, before and after extracellular intervention to alter cell ion concentration. Given widespread availability of ζ-potential measurement apparatus (most typically in chemistry and materials science settings), this offers a new method of measuring V m without the need for fluorescence measurements or calibration curves.",

keywords = "action potential, excitable cell, H9c2 cardiomyoblasts, RMP, zeta potential",

author = "Stephanie Chacar and Catacutan, {Mary Krystelle} and Shamma Albakr and {Al Safar}, Habiba and Samira Babiker and Samar Ahmed and Albizreh, {Anas A.} and Alshehhi, {Ahmed Z.} and Sungmun Lee and Moni Nader and Hughes, {Michael Pycraft}",

note = "Publisher Copyright: {\textcopyright} 2024 The Author(s). Published by IOP Publishing Ltd.",

year = "2024",

month = may,

doi = "10.1088/1361-6501/ad25de",

language = "British English",

volume = "35",

journal = "Measurement Science and Technology",

issn = "0957-0233",

publisher = "IOP Publishing Ltd.",

number = "5",

}

Chacar, S, Catacutan, MK, Albakr, S, Al Safar, H, Babiker, S, Ahmed, S, Albizreh, AA, Alshehhi, AZ, Lee, S, Nader, M 2024, 'Rapid, label-free, contactless measurement of membrane potential in excitable H9c2 cardiomyoblasts using ζ-potential', Measurement Science and Technology, vol. 35, no. 5, 055701. https://doi.org/10.1088/1361-6501/ad25de

Rapid, label-free, contactless measurement of membrane potential in excitable H9c2 cardiomyoblasts using ζ-potential. / Chacar, Stephanie; Catacutan, Mary Krystelle; Albakr, Shamma et al.
In: Measurement Science and Technology, Vol. 35, No. 5, 055701, 05.2024.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Rapid, label-free, contactless measurement of membrane potential in excitable H9c2 cardiomyoblasts using ζ-potential

AU - Chacar, Stephanie

AU - Catacutan, Mary Krystelle

AU - Albakr, Shamma

AU - Al Safar, Habiba

AU - Babiker, Samira

AU - Ahmed, Samar

AU - Albizreh, Anas A.

AU - Alshehhi, Ahmed Z.

AU - Lee, Sungmun

AU - Nader, Moni

AU - Hughes, Michael Pycraft

N1 - Publisher Copyright:© 2024 The Author(s). Published by IOP Publishing Ltd.

PY - 2024/5

Y1 - 2024/5

N2 - The measurement of cell membrane potential (V m) is important for understanding ion channel function. V m plays a role in several routine cellular functions and diseases, particularly in excitable cells such as muscle and nerve. However, measuring V m is difficult, relying either on labour-intensive direct measurement of single cells (intracellular electrodes, patch clamp) or indirect measurement of fluorescence intensity, using V m-sensitive labels. Here we demonstrate a direct measurement technique based on determination of the cell’s ζ-potential, the electrical potential at the hydrodynamic shear plane, approximately 1 nm beyond the cell surface. We demonstrate this principle using excitable H9c2 cardiomyoblasts, measured in both polarised and depolarised states, before and after extracellular intervention to alter cell ion concentration. Given widespread availability of ζ-potential measurement apparatus (most typically in chemistry and materials science settings), this offers a new method of measuring V m without the need for fluorescence measurements or calibration curves.

AB - The measurement of cell membrane potential (V m) is important for understanding ion channel function. V m plays a role in several routine cellular functions and diseases, particularly in excitable cells such as muscle and nerve. However, measuring V m is difficult, relying either on labour-intensive direct measurement of single cells (intracellular electrodes, patch clamp) or indirect measurement of fluorescence intensity, using V m-sensitive labels. Here we demonstrate a direct measurement technique based on determination of the cell’s ζ-potential, the electrical potential at the hydrodynamic shear plane, approximately 1 nm beyond the cell surface. We demonstrate this principle using excitable H9c2 cardiomyoblasts, measured in both polarised and depolarised states, before and after extracellular intervention to alter cell ion concentration. Given widespread availability of ζ-potential measurement apparatus (most typically in chemistry and materials science settings), this offers a new method of measuring V m without the need for fluorescence measurements or calibration curves.

KW - action potential

KW - excitable cell

KW - H9c2 cardiomyoblasts

KW - RMP

KW - zeta potential

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U2 - 10.1088/1361-6501/ad25de

DO - 10.1088/1361-6501/ad25de

M3 - Article

AN - SCOPUS:85184991445

SN - 0957-0233

VL - 35

JO - Measurement Science and Technology

JF - Measurement Science and Technology

IS - 5

M1 - 055701

ER -

Chacar S, Catacutan MK, Albakr S, Al Safar H, Babiker S, Ahmed S et al. Rapid, label-free, contactless measurement of membrane potential in excitable H9c2 cardiomyoblasts using ζ-potential. Measurement Science and Technology. 2024 May;35(5):055701. doi: 10.1088/1361-6501/ad25de